解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Ion channel models are the building blocks of computational neuron models. Their biological fidelity is therefore crucial for the interpretation of simulations. However, the number of published models, and the lack of standardization, make the comparison of ion channel models with one another and with experimental dat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22152
更新日期:2017-03-06 00:00:00
abstract::Apical constriction is a widely utilized cell shape change linked to folding, bending and invagination of polarized epithelia. It remains unclear how apical constriction is regulated spatiotemporally during tissue invagination and how this cellular process contributes to tube formation in different developmental conte...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22235
更新日期:2017-03-06 00:00:00
abstract::Unfavorable redox conditions in the endoplasmic reticulum (ER) can decrease the capacity for protein secretion, altering vital cell functions. While systems to manage reductive stress are well-established, how cells cope with an overly oxidizing ER remains largely undefined. In previous work (Wang et al., 2014), we de...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.24141
更新日期:2017-03-03 00:00:00
abstract::Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22086
更新日期:2017-02-28 00:00:00
abstract::The Frank-Starling relation is a fundamental auto-regulatory property of the heart that ensures the volume of blood ejected in each heartbeat is matched to the extent of venous filling. At the cellular level, heart muscle cells generate higher force when stretched, but despite intense efforts the underlying molecular ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.24081
更新日期:2017-02-23 00:00:00
abstract::The segregation of the trophectoderm (TE) from the inner cell mass (ICM) in the mouse blastocyst is determined by position-dependent Hippo signaling. However, the window of responsiveness to Hippo signaling, the exact timing of lineage commitment and the overall relationship between cell commitment and global gene exp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22906
更新日期:2017-02-22 00:00:00
abstract::Squamous cell carcinomas occurring at transition zones are highly malignant tumors with poor prognosis. The identity of the cell population and the signaling pathways involved in the progression of transition zone squamous cell carcinoma are poorly understood, hence representing limited options for targeted therapies....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22914
更新日期:2017-02-21 00:00:00
abstract::Manipulating gene function cell type-specifically is a common experimental goal in Drosophila research and has been central to studies of neural development, circuit computation, and behavior. However, current cell type-specific gene disruption techniques in flies often reduce gene activity incompletely or rely on cel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22279
更新日期:2017-02-17 00:00:00
abstract::Most evolutionary thinking is based on the notion of fitness and related ideas such as fitness landscapes and evolutionary optima. Nevertheless, it is often unclear what fitness actually is, and its meaning often depends on the context. Here we argue that fitness should not be a basal ingredient in verbal or mathemati...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23804
更新日期:2017-02-15 00:00:00
abstract::Serotonin (5-HT) is associated with mood and motivation but the function of endogenous 5-HT remains controversial. Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time scales from seconds to weeks. We found that activating dorsal raphe nucleus (DRN) 5-HT neurons induced a stro...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20975
更新日期:2017-02-14 00:00:00
abstract::Plasmodium falciparum causes malaria in humans with over 450,000 deaths annually. The asexual blood stage involves invasion of erythrocytes by merozoites, in which they grow and divide to release daughter merozoites, which in turn invade new erythrocytes perpetuating the cycle responsible for malaria. A key step in me...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21347
更新日期:2017-02-14 00:00:00
abstract::Strong kinetochore-microtubule attachments are essential for faithful segregation of sister chromatids during mitosis. The Dam1 and Ndc80 complexes are the main microtubule binding components of the Saccharomyces cerevisiae kinetochore. Cooperation between these two complexes enhances kinetochore-microtubule coupling ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21069
更新日期:2017-02-13 00:00:00
abstract::Pathological metabolic conditions such as ischemia induce the rupture of the mitochondrial envelope and the release of pro-apoptotic proteins, leading to cell death. At the onset of this process, the inner mitochondrial membrane becomes depolarized and permeable to osmolytes, proposedly due to the opening of a non-sel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23781
更新日期:2017-02-10 00:00:00
abstract::In addition to their role in desensitization and internalization of G protein-coupled receptors (GPCRs), β-arrestins are essential scaffolds linking GPCRs to Erk1/2 signaling. However, their role in GPCR-operated Erk1/2 activation differs between GPCRs and the underlying mechanism remains poorly characterized. Here, w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23777
更新日期:2017-02-07 00:00:00
abstract::Although seasonality is widespread and can cause fluctuations in the intensity and direction of natural selection, we have little information about the consequences of seasonal fitness trade-offs for population dynamics. Here we exposed populations of Drosophila melanogaster to repeated seasonal changes in resources a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18770
更新日期:2017-02-06 00:00:00
abstract::A small molecule called Sm4 can disrupt interactions involving a transcription factor called Sox18, while having little impact on other members of the SoxF family. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.24238
更新日期:2017-01-31 00:00:00
abstract::Adult-born neurons are continually produced in the dentate gyrus but it is unclear whether synaptic integration of new neurons affects the pre-existing circuit. Here we investigated how manipulating neurogenesis in adult mice alters excitatory synaptic transmission to mature dentate neurons. Enhancing neurogenesis by ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19886
更新日期:2017-01-30 00:00:00
abstract::The number of ions required to drive substrate transport through a secondary active transporter determines the protein's ability to create a substrate gradient, a feature essential to its physiological function, and places fundamental constraints on the transporter's mechanism. Stoichiometry is known for a wide array ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21016
更新日期:2017-01-25 00:00:00
abstract::RNA molecules cause the proteins involved in the formation of germ granules to coalesce into liquid droplets. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.24106
更新日期:2017-01-24 00:00:00
abstract::Genomic sequencing has implicated large numbers of genes and de novo mutations as potential disease risk factors. A high throughput in vivo model system is needed to validate gene associations with pathology. We developed a Drosophila-based functional system to screen candidate disease genes identified from Congenital...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22617
更新日期:2017-01-20 00:00:00
abstract::In 2015, as part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Kandela et al., 2015) that described how we intended to replicate selected experiments from the paper "BET bromodomain inhibition as a therapeutic strategy to target c-Myc" (Delmore et al., 2011). Here we report the resu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21253
更新日期:2017-01-19 00:00:00
abstract::Rhabdomyosarcoma (RMS) is a pediatric malignacy of muscle with myogenic regulatory transcription factors MYOD and MYF5 being expressed in this disease. Consensus in the field has been that expression of these factors likely reflects the target cell of transformation rather than being required for continued tumor growt...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19214
更新日期:2017-01-12 00:00:00
abstract::Pregnancy complications are poorly represented in the archeological record, despite their importance in contemporary and ancient societies. While excavating a Byzantine cemetery in Troy, we discovered calcified abscesses among a woman's remains. Scanning electron microscopy of the tissue revealed 'ghost cells', result...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20983
更新日期:2017-01-10 00:00:00
abstract::Glucose-6-phosphate dehydrogenase (G6PD) deficiency is believed to confer protection against Plasmodium falciparum malaria, but the precise nature of the protective effecthas proved difficult to define as G6PD deficiency has multiple allelic variants with different effects in males and females, and it has heterogeneou...
journal_title:eLife
pub_type: 杂志文章,多中心研究
doi:10.7554/eLife.15085
更新日期:2017-01-09 00:00:00
abstract::Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however, current understanding of host-microbiome interactions derives mostly from studies in which elucidation of microbe-microbe interactions is difficult. In exploring how Drosophila melanogas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18855
更新日期:2017-01-09 00:00:00
abstract::Visual perception and behavior are mediated by cortical areas that have been distinguished using architectonic and retinotopic criteria. We employed fluorescence imaging and GCaMP6 reporter mice to generate retinotopic maps, revealing additional regions of retinotopic organization that extend into barrel and retrosple...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18372
更新日期:2017-01-06 00:00:00
abstract::hIAPP fibrils are associated with Type-II Diabetes, but the link of hIAPP structure to islet cell death remains elusive. Here we observe that hIAPP fibrils are cytotoxic to cultured pancreatic β-cells, leading us to determine the structure and cytotoxicity of protein segments composing the amyloid spine of hIAPP. Usin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19273
更新日期:2017-01-03 00:00:00
abstract::Experience-dependent reorganisation of functional maps in the cerebral cortex is well described in the primary sensory cortices. However, there is relatively little evidence for such cortical reorganisation over the short-term. Using human somatosensory cortex as a model, we investigated the effects of a 24 hr gluing ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17280
更新日期:2016-12-30 00:00:00
abstract::Understanding how fungi specialize on their plant host is crucial for developing sustainable disease control. A traditional, centuries-old rice agro-system of the Yuanyang terraces was used as a model to show that virulence effectors of the rice blast fungus Magnaporthe oryzaeh play a key role in its specialization on...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19377
更新日期:2016-12-23 00:00:00
abstract::Mitochondria support synaptic transmission through production of ATP, sequestration of calcium, synthesis of glutamate, and other vital functions. Surprisingly, less than 50% of hippocampal CA1 presynaptic boutons contain mitochondria, raising the question of whether synapses without mitochondria can sustain changes i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15275
更新日期:2016-12-19 00:00:00
abstract::The spinal cord has the capacity to coordinate motor activities such as locomotion. Following spinal transection, functional activity can be regained, to a degree, following motor training. To identify microcircuits involved in this recovery, we studied a population of mouse spinal interneurons known to receive direct...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21715
更新日期:2016-12-15 00:00:00
abstract::Seed dormancy is a complex life history trait that determines the timing of germination and is crucial for local adaptation. Genetic studies of dormancy are challenging, because the trait is highly plastic and strongly influenced by the maternal environment. Using a combination of statistical and experimental approach...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22502
更新日期:2016-12-14 00:00:00
abstract::Two-component systems (TCS) are protein machineries that enable cells to respond to input signals. Histidine kinases (HK) are the sensory component, transferring information toward downstream response regulators (RR). HKs transfer phosphoryl groups to their specific RRs, but also dephosphorylate them, overall ensuring...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21422
更新日期:2016-12-12 00:00:00
abstract::The brain has an impressive ability to withstand neural damage. Diseases that kill neurons can go unnoticed for years, and incomplete brain lesions or silencing of neurons often fail to produce any behavioral effect. How does the brain compensate for such damage, and what are the limits of this compensation? We propos...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12454
更新日期:2016-12-09 00:00:00
abstract::Lipoprotein lipase (LPL) undergoes spontaneous inactivation via global unfolding and this unfolding is prevented by GPIHBP1 (Mysling et al., 2016). We now show: (1) that ANGPTL4 inactivates LPL by catalyzing the unfolding of its hydrolase domain; (2) that binding to GPIHBP1 renders LPL largely refractory to this inhib...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20958
更新日期:2016-12-08 00:00:00
abstract::Accurate protein localization is crucial to generate and maintain organization in all cells. Achieving accuracy is challenging, as the molecular signals that dictate a protein's cellular destination are often promiscuous. A salient example is the targeting of an essential class of tail-anchored (TA) proteins, whose so...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21301
更新日期:2016-12-07 00:00:00
abstract::Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19850
更新日期:2016-12-06 00:00:00
abstract::The twin-arginine protein translocation system (Tat) transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts. The Tat transporter is assembled from multiple copies of the membrane proteins TatA, TatB, and TatC. We combine sequence co-evolution analysis, mo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20718
更新日期:2016-12-03 00:00:00
abstract::Variation in the presence or absence of transposable elements (TEs) is a major source of genetic variation between individuals. Here, we identified 23,095 TE presence/absence variants between 216 Arabidopsis accessions. Most TE variants were rare, and we find these rare variants associated with local extremes of gene ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20777
更新日期:2016-12-02 00:00:00
abstract::Migration of skeletal muscle precursor cells is a key step during limb muscle development and depends on the activity of PAX3 and MET. Here, we demonstrate that BRAF serves a crucial function in formation of limb skeletal muscles during mouse embryogenesis downstream of MET and acts as a potent inducer of myoblast cel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18351
更新日期:2016-12-01 00:00:00